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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Mitochondria 

Staining summary

Staining of cytoplasm and plasma membrane in all three cell lines with additional staining of nucleus and cytoplasm in U-251 MG and U-2 OS.

Reliability (Single)

Antibodies in assay

HPA035533
 

Antibody HPA035533

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm, Mitochondria 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli, plasma membrane, cytoplasm & mitochondria.
 

Summary

Staining of cytoplasm and plasma membrane in all three cell lines with additional staining of nucleus and cytoplasm in U-251 MG and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Mitochondria
Nucleus but not nucleoli
Plasma membrane