Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Cytoplasm, Cytoskeleton (Intermediate filaments), Cytoskeleton (Microtubules)

Additional location(s)

 

Staining summary

Localized to the cytoplasm, microtubules and intermediate filaments.

Reliability (APE)

Very low

Antibodies in assay

HPA007982, HPA011933
 

Antibody HPA007982

 

Antibody HPA011933

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Cytoplasm, Cytoskeleton (Microtubules)  

Staining characteristics

Granular  

Staining intensity

Weak Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
No staining.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Cytoplasm, Cytoskeleton (Microtubules) Cytoskeleton (Intermediate filaments), Aggresome 

Staining characteristics

Granular  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Cytoplasm, Cytoskeleton (Microtubules) Cytoskeleton (Intermediate filaments) 

Staining characteristics

Granular Granular 

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & cytoskeleton (microtubules).
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments) & aggresome.
 

Summary

Granular staining of cytoskeleton (microtubules) and cytoplasm in all three cell lines.   Staining of cytoskeleton (intermediate filaments) in U-251 MG and U-2 OS. Additional staining of agressomes in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Intermediate filaments
Microtubules