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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus, Nucleoli, Plasma membrane 

Staining summary

Staining of cytoplasm in all three cell lines. Additional staining of nucleoli and plasma membrane in A-431 and nucleus and cytoplasm in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA029109
 

Antibody HPA029109

 

         Cell line A-431

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Location(s)

Nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus, Nucleoli, Cytoplasm 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleoli, plasma membrane & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines. Additional staining of nucleoli and plasma membrane in A-431 and nucleus and cytoplasm in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleoli
Nucleus
Plasma membrane