ADCK4

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA027229

 

Antibody HPA027277

 

Antibody HPA027279

 

Antibody HPA028303

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA027229 HPA027277 HPA027279 HPA028303 

Host species

Rabbit Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 6 6 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment 

Length (aa)

65 87 61 80 

Antigen sequence

ACAQNFRQLLANDPFFRVPAVVKELCTTRVLGMELAGGVPLDQCQGLSQD
LRNQICFQLLTLCLR
 
HQVTLLDFGASREFGTEFTDHYIEVVKAAADGDRDCVLQKSRDLKFLTGF
ETKAFSDAHVEAVMILGEPFATQGPYDFGSGETARRI
 
RQSADFMPRWQMLRVLEEELGRDWQAKVASLEEVPFAAASIGQVHQGLLR
DGTEVAVKIQY
 
GTGGQLGQTVGWPCGALGPGPHRWGPCGGSWAQKFYQDGPGRGLGEEDIR
RAREARPRKTPRPQLSDRSRERKVPASRIS
 

Matching transcripts

ADCK4-001 - ENSP00000315118 [100%]
ADCK4-002 - ENSP00000243583 [100%]
 ADCK4-001 - ENSP00000315118 [100%]
ADCK4-002 - ENSP00000243583 [100%]
 ADCK4-001 - ENSP00000315118 [100%]
ADCK4-002 - ENSP00000243583 [100%]
ADCK4-014 - ENSP00000470894 [100%]
ADCK4-018 - ENSP00000470916 [84%]
 ADCK4-001 - ENSP00000315118 [100%]
ADCK4-002 - ENSP00000243583 [100%]
ADCK4-006 - ENSP00000471310 [100%]
ADCK4-009 - ENSP00000470876 [100%]
ADCK4-010 - ENSP00000472978 [100%]
ADCK4-012 - ENSP00000473189 [100%]
ADCK4-013 - ENSP00000472925 [100%]
ADCK4-018 - ENSP00000470916 [100%]
 

Other gene match

    

ANTIBODY VALIDATION

         Immunohistochemistry

Image

    

Description

Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity and distinct staining of luminal membranes in tubular cells.
More information
 Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6 HIER pH6 HIER pH6  

Antibody dilution

1:25 1:500 1:1000  

Literature conformity

Partly consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data  

RNA consistency

Not done Not done Not done  

         Immunofluorescence

Image

    

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
More information
 Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
More information
 Immunofluorescent staining of human cell line A549 shows positivity in mitochondria.
More information
 

Antibody dilution

1:100  1:200 1:35 

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available.  Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

    

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403036)
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403036)
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

60.1, 55.9 60.1, 55.9 60.1, 55.9, 25.0, 22.5 60.1, 55.9, 25.0, 16.5, 15.8, 14.4, 12.9, 11.8 

Antibody dilution

1:250 1:250 1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

    

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:12000 1:30000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
ADCK4-001
 
ADCK4-002
 
ADCK4-006
 
ADCK4-009
 
ADCK4-010
 
ADCK4-012
 
ADCK4-013
 
ADCK4-014
 
ADCK4-018