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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm, Mitochondria

Additional location(s)

Nucleus, Plasma membrane 

Staining summary

Mainly localized to mitochondria and the cytoplasm. Also localized to the nucleus and plasma membrane.

Reliability (APE)

Very low

Antibodies in assay

HPA027229, HPA027277, HPA027279
 

Antibody HPA027229

 

Antibody HPA027277

 

Antibody HPA027279

 

         Cell line A-431

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Location(s)

Mitochondria Nucleus but not nucleoli, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

Granular   

Staining intensity

Weak Strong Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Mitochondria Nucleus, Mitochondria Plasma membrane 

Staining characteristics

   

Staining intensity

Weak Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

 Granular  

Staining intensity

Negative Weak Strong 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & mitochondria.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of mitochondria in A-431 and U-2 OS.   Staining of nucleus in all three cell lines with additional staining of mitochondria in U-2 OS and cytoplasm in U-251 and A-431.   Staining of plasma membrane in all three cell lines with additional stainind of cytoplasm in U-251 MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Mitochondria
Nucleus
Plasma membrane