GLO1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody CAB040541

 

Antibody CAB040542

 

ANTIBODY INFORMATION

Provider

NCI-CPTC
 NCI-CPTC
 

Product name

CPTC-GLO1-1 CPTC-GLO1-3 

Host species

Mouse Mouse 

Clonality

mAb mAb 

Purity

Protein A/G Protein A/G 

Released in version

7 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein Recombinant protein 

Length (aa)

  

Antigen sequence

 
 

Matching transcripts

  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human prostate shows strong nuclear, cytoplasmic and membranous positivity in glandular cells along with distinct extracellular material.
More information
 Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in leydig cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:3000 1:20000 

Validation IH

Uncertain: Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data Uncertain: Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
More information
 Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
More information
 

Antibody dilution

1:50 1:200 

Validation IF

Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

20.8 20.8 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 
 

ANTIGEN VIEW

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 Not available