Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm and nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA003563, CAB004209, HPA020432
 

Antibody HPA003563

 

Antibody CAB004209

 

Antibody HPA020432

 

         Cell line A-431

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Location(s)

  Cytoplasm 

Staining characteristics

   

Staining intensity

Negative Negative Moderate 

Validation

No staining.
 
No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Cell line U-2 OS

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Location(s)

  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

   

Staining intensity

Negative Negative Moderate 

Validation

No staining.
 
No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Cytoplasm  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular  Granular 

Staining intensity

Weak Negative Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Granular staining of cytoplasm in U-251MG.   No staining was observed in any of the three cell lines.   Staining of cytoplasm in all three cell lines with additional staining of nucleus in U-251 MG and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli