Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Plasma membrane, Cytoplasm 

Staining summary

Staining of cytoplasm and speckled nucleus in all three cell lines with additional staining of plasma membrane in U-251 MG and U-2 OS.

Reliability (Single)

Antibodies in assay

HPA000996
 

Antibody HPA000996

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Speckle 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

Granular, Speckle 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

Granular, Speckle 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm and speckled nucleus in all three cell lines with additional staining of plasma membrane in U-251 MG and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Plasma membrane