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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Centrosome

Additional location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining summary

Staining of centrosome and plasma membrane in all three cell lines with additional staining of nucleus in U-251 MG and A-431 and cytoplasm in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA002861
 

Antibody HPA002861

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Centrosome 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm, Centrosome 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Centrosome 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane & centrosome.
 

Summary

Staining of centrosome and plasma membrane in all three cell lines with additional staining of nucleus in U-251 MG and A-431 and cytoplasm in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Nucleus but not nucleoli
Plasma membrane