The cancer tissue page shows antibody staining in 20 different cancers. The assay and annotation is described here.
This page starts with information about the protein evidence and, when applicable, also protein class. Below the summary, a selection of four standard cancer tissue samples is displayed as representative of the overall staining pattern. From left: colorectal cancer, breast cancer, prostate cancer and lung cancer. An additional 5th image can be added as a complement.
The cancer tissue summary page shows antibody staining in 20 different cancers. The assay and annotation is described here.
For each cancer, the fraction of samples with antibody staining/protein expression level high, medium, low, or not detected are provided by the blue-scale color-coding (as described by the color-coding scale in the box to the right). The length of the bar represents the number of patient samples analyzed (max=12 patients). The images and annotations can be accessed by clicking on the cancer name or protein expression bar. If more than one antibody is analyzed, the tabs at the top of the staining summary section can be used to toggle between the different antibodies. The tooltip function displays additional data for the features in the staining summary view.
Next to the cancer staining data, the protein expression data of normal tissues or specific cell types corresponding to each cancer are shown and protein expression levels are indicated by the blue-scale color coding.
At the bottom of the page, a summary of the overall protein expression pattern across the analyzed cancer tissues as well as information about literature conformity are presented.
Several hepatocellular carcinomas, urothelial cancers and melanomas along with a few gliomas and colorectal cancers displayed weak to moderate cytoplasmic and/or nuclear positivity. Cases of lung adenocarcinomas showed moderate to strong staining. Remaining cancers were negative.
Strong cytoplasmic and nuclear immunoreactivity was observed in lymphomas and cases of melanomas and breast cancers. Remaining cancers were in general negative.
Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.
STAT5A (HGNC Symbol)
Signal transducer and activator of transcription 5A (HGNC Symbol)
Entrez gene summary
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.
At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.
Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50) (read more).
If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.
Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.
The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.
The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.
The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.
The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.
P42229 [Direct mapping] Signal transducer and activator of transcription 5A
Predicted intracellular proteins Plasma proteins Transcription factors Immunoglobulin fold Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
GO:0000255 [allantoin metabolic process] GO:0000979 [RNA polymerase II core promoter sequence-specific DNA binding] GO:0001553 [luteinization] GO:0001779 [natural killer cell differentiation] GO:0003677 [DNA binding] GO:0003700 [transcription factor activity, sequence-specific DNA binding] GO:0004871 [signal transducer activity] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0006101 [citrate metabolic process] GO:0006103 [2-oxoglutarate metabolic process] GO:0006105 [succinate metabolic process] GO:0006107 [oxaloacetate metabolic process] GO:0006351 [transcription, DNA-templated] GO:0006355 [regulation of transcription, DNA-templated] GO:0006357 [regulation of transcription from RNA polymerase II promoter] GO:0006549 [isoleucine metabolic process] GO:0006573 [valine metabolic process] GO:0006600 [creatine metabolic process] GO:0006631 [fatty acid metabolic process] GO:0007165 [signal transduction] GO:0007259 [JAK-STAT cascade] GO:0007565 [female pregnancy] GO:0007595 [lactation] GO:0008284 [positive regulation of cell proliferation] GO:0018108 [peptidyl-tyrosine phosphorylation] GO:0019218 [regulation of steroid metabolic process] GO:0019221 [cytokine-mediated signaling pathway] GO:0019530 [taurine metabolic process] GO:0019915 [lipid storage] GO:0030155 [regulation of cell adhesion] GO:0030856 [regulation of epithelial cell differentiation] GO:0030879 [mammary gland development] GO:0032825 [positive regulation of natural killer cell differentiation] GO:0033026 [negative regulation of mast cell apoptotic process] GO:0033077 [T cell differentiation in thymus] GO:0038161 [prolactin signaling pathway] GO:0040014 [regulation of multicellular organism growth] GO:0040018 [positive regulation of multicellular organism growth] GO:0042104 [positive regulation of activated T cell proliferation] GO:0043029 [T cell homeostasis] GO:0043066 [negative regulation of apoptotic process] GO:0043434 [response to peptide hormone] GO:0045086 [positive regulation of interleukin-2 biosynthetic process] GO:0045579 [positive regulation of B cell differentiation] GO:0045588 [positive regulation of gamma-delta T cell differentiation] GO:0045621 [positive regulation of lymphocyte differentiation] GO:0045647 [negative regulation of erythrocyte differentiation] GO:0045931 [positive regulation of mitotic cell cycle] GO:0045944 [positive regulation of transcription from RNA polymerase II promoter] GO:0045954 [positive regulation of natural killer cell mediated cytotoxicity] GO:0046449 [creatinine metabolic process] GO:0046543 [development of secondary female sexual characteristics] GO:0046544 [development of secondary male sexual characteristics] GO:0048541 [Peyer's patch development] GO:0050729 [positive regulation of inflammatory response] GO:0060376 [positive regulation of mast cell differentiation] GO:0060397 [JAK-STAT cascade involved in growth hormone signaling pathway] GO:0060740 [prostate gland epithelium morphogenesis] GO:0060742 [epithelial cell differentiation involved in prostate gland development] GO:0061180 [mammary gland epithelium development] GO:0070668 [positive regulation of mast cell proliferation] GO:0097011 [cellular response to granulocyte macrophage colony-stimulating factor stimulus]