Fields »

SUBCELLULAR LOCATION

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Main location(s)

Golgi apparatus

Additional location(s)

Vesicles, Centrosome 

Staining summary

Mainly localized to the Golgi apparatus. In addition localized to centrosome or vesicles.

Reliability (APE)

High

Antibodies in assay

HPA008548, CAB012909, HPA026109
 

Antibody HPA008548

 

Antibody CAB012909

 

Antibody HPA026109

 

         Cell line A-431

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Location(s)

Golgi apparatus, Vesicles, Centrosome Nucleus but not nucleoli, Cytoplasm, Golgi apparatus Golgi apparatus 

Staining characteristics

   

Staining intensity

Moderate Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Golgi apparatus, Vesicles Golgi apparatus Golgi apparatus 

Staining characteristics

   

Staining intensity

Moderate Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Golgi apparatus, Centrosome Golgi apparatus Golgi apparatus 

Staining characteristics

   

Staining intensity

Strong Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in golgi apparatus & centrosome.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in golgi apparatus.
 

Immunofluorescent staining of human cell line A-431 shows positivity in golgi apparatus.
 

Summary

Staining of the Golgi apparatus and/or centrosome and vesicles in all three cell lines.   Staining of the Golgi apparatus in all three cell lines with additional staining of nucleus and cytoplasm in A-431.   Staining of the Golgi apparatus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Centrosome
Golgi apparatus
Endosome