DCTD

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA035894

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA035894 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

91 

Antigen sequence

QPCGPNMSEVSCKKRDDYLEWPEYFMAVAFLSAQRSKDPNSQVGACIVNS
ENKIVGIGYNGMPNGCSDDVLPWRRTAENKLDTKYPYVCHA
 

Matching transcripts

DCTD-001 - ENSP00000349576 [100%]
DCTD-002 - ENSP00000398194 [93%]
DCTD-006 - ENSP00000422662 [93%]
DCTD-007 - ENSP00000423182 [93%]
DCTD-009 - ENSP00000424017 [93%]
DCTD-015 - ENSP00000424050 [93%]
DCTD-016 - ENSP00000423894 [93%]
DCTD-014 - ENSP00000421792 [80%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:100 

Validation IH

Uncertain: One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:77 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

21.0, 20.0, 19.0, 13.4, 11.0, 8.2 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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DCTD-001
 
DCTD-002
 
DCTD-006
 
DCTD-007
 
DCTD-009
 
DCTD-014
 
DCTD-015
 
DCTD-016