MPDU1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA014845

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA014845 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

42 

Antigen sequence

AEADGPLKRLLVPILLPEKCYDQLFVQWDLLHVPCLKILLSK
 

Matching transcripts

MPDU1-001 - ENSP00000250124 [100%]
MPDU1-006 - ENSP00000414071 [100%]
MPDU1-007 - ENSP00000458741 [100%]
MPDU1-009 - ENSP00000459498 [100%]
MPDU1-010 - ENSP00000459306 [100%]
MPDU1-019 - ENSP00000463056 [100%]
MPDU1-020 - ENSP00000462229 [100%]
MPDU1-022 - ENSP00000379758 [100%]
MPDU1-023 - ENSP00000464524 [100%]
MPDU1-024 - ENSP00000462839 [100%]
MPDU1-026 - ENSP00000464158 [100%]
MPDU1-027 - ENSP00000352876 [100%]
MPDU1-015 - ENSP00000459409 [95%]
MPDU1-025 - ENSP00000463037 [95%]
MPDU1-016 - ENSP00000460864 [86%]
MPDU1-011 - ENSP00000461183 [84%]
MPDU1-028 - ENSP00000463966 [81%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:400 

Validation IH

Uncertain: One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line A-431 shows positivity in endoplasmic reticulum.
More information
 

Antibody dilution

1:167 

Validation IF

Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

26.6, 20.9, 20.8, 19.8, 16.7, 16.5, 12.7, 11.8, 11.0, 8.2, 6.5, 5.7 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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MPDU1-001
 
MPDU1-006
 
MPDU1-007
 
MPDU1-009
 
MPDU1-010
 
MPDU1-011
 
MPDU1-015
 
MPDU1-016
 
MPDU1-019
 
MPDU1-020
 
MPDU1-022
 
MPDU1-023
 
MPDU1-024
 
MPDU1-025
 
MPDU1-026
 
MPDU1-027
 
MPDU1-028
 
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