Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm 

Staining summary

Staining of nuclei and cytoplasm in all three cell lines.

Reliability (Single)

Antibodies in assay

HPA001140
 

Antibody HPA001140

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nuclei and cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli