The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.
Immunohistochemical staining of human pancreas shows distinct positivity in acinar luminal membranes.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic and strong luminal membranous positivity in exocrine cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human pancreas shows moderate granular positivity in exocrine cells.
Summary
Most normal tissues displayed weak cytoplasmic and membranous positivity or were negative. Moderate neuropil positivity in cerebellum. Trophoblastic cells, intercalated ducts in pancreas, bile ducts, thyroid and smooth muscle showed moderate to strong positivity.
Most of the normal tissues displayed weak to moderate cytoplasmic positivity accompanied with membranous immunoreactivity. Nuclear staining was observed in for example lung alveolar cells type 2 and squamous epithelia. Cells of red pulp of spleen and bone marrow poietic cells showed strong positivity.
Most of the normal tissues displayed moderate cytoplasmic immunoreactivity, often combined with membranous positivity. Hepatocytes, glial cells, ovarian and endometrial stroma were weakly stained or negative.
Most of the normal tissues displayed weak to moderate cytoplasmic positivity. Cells in seminiferus ducts and subsets of neuronal cells were strongly stained. Skin, lymphoid tissues, myocytes and pancreatic islets were negative.
Most normal tissues displayed moderate to strong cytoplasmic immunoreactivity, often with a granular pattern. Strongest staining was observed in endocrine glands and the male genital tract.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
