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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Golgi apparatus, Centrosome

Additional location(s)

 

Staining summary

Localized to plasma membrane, the Golgi apparatus and centrosomes.

Reliability (APE)

Very low

Antibodies in assay

CAB017781, HPA019693
 

Antibody CAB017781

 

Antibody HPA019693

 

         Cell line A-431

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Location(s)

Centrosome   

Staining characteristics

   

Staining intensity

Weak   

Validation

Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
   

Comment

   

         Cell line U-2 OS

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Location(s)

   

Staining characteristics

   

Staining intensity

Negative   

Validation

No staining.
   

Comment

   

         Cell line U-251 MG

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Location(s)

Centrosome Plasma membrane, Golgi apparatus 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in centrosome.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane & golgi apparatus.
 

Summary

Staining of centrosome in A-431 and U-251 MG.   Staining of plasma membrane and Golgi apparatus in U-251 MG.  

Validation

The subcellular location is not consistent with literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Golgi apparatus
Plasma membrane