Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Plasma membrane, Cytoplasm 

Staining summary

Staining of nucleus, cytoplasm and plasma membrane in all three cell lines.

Reliability (Single)

Antibodies in assay

HPA042266
 

Antibody HPA042266

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
 

Summary

Staining of nucleus, cytoplasm and plasma membrane in all three cell lines.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Plasma membrane