The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human rectum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human oral mucosa shows strong nucleolar positivity in squamous epithelial cells.
Strong cytoplasmic immunoreactivity with a granular pattern was observed in most normal tissues. The gall bladder, pancreas, thyroid and prostate glands displayed moderate staining whereas hepatocytes and most neuronal cells were weakly positive. Glomeruli and bile duct cells were negative.
Normal tissues generally showed moderate nucleolar staining which in several cases was combined with cytoplasmic positivity. Strong cytoplasmic staining was observed in hepatocytes and renal tubules. The large intestine and pneumocytes showed strong membranous staining. Bile duct cells, islets of Langerhans, renal glomeruli, spleen and glial cells were weakly stained or negative.
The multi-targeting antibody yielding a staining pattern partly consistent with characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein chara
One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data