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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm, Centrosome 

Staining summary

Mainly localized to the nucleus.

Reliability (APE)

Low

Antibodies in assay

HPA014232, HPA043265
 

Antibody HPA014232

 

Antibody HPA043265

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Centrosome Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Centrosome Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Moderate Moderate 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Centrosome Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & centrosome.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of centrosome and nucleus in all three cell lines. Additional staining of cytoplasm in A-431 and U-2 OS.   Staining of nucleus and cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Centrosome
Cytoplasm
Nucleus but not nucleoli