The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human lymph node shows distinct cytoplasmic and nuclear positivity in cells outside the reaction centra.
Immunohistochemical staining of human lymph node shows strong cytoplasmic and nuclear positivity in lymphoid cells outside reaction centra.
Immunohistochemical staining of human lymph node shows strong positivity in subsets of cells outside the germinal center.
Summary
Alveolar macrophages and a subset of cells in lymphoid tissues exhibited strong cytoplasmic and nuclear staining. Weak to moderate cytoplasmic staining was observed in gastric glands, gall bladder, exocrine pancreas and testis while most other normal tissues were negative.
A small subset of lymphoid cells in lymph node, tonsil spleen and gastrointestinal tract showed strong cytoplasmic staining. distinct staining was also seen in skeletal muscle. Other tissues and cells were negative.
Weak to moderate cytoplasmic positivity was observed in most normal tissues. Subsets of lymphoid cells, hepatocytes and gall bladder were strongly stained. Glial cells and lymphoid reaction center cells were basically negative.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
