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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Mitochondria, Vesicles, Cytoskeleton (Microtubules)

Additional location(s)

 

Staining summary

Localized to microtubules, mitochondria or nucleus and vesicles.

Reliability (APE)

Very low

Antibodies in assay

HPA023072, HPA023149, HPA027772
 

Antibody HPA023072

 

Antibody HPA023149

 

Antibody HPA027772

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Vesicles Mitochondria Cytoskeleton (Microtubules) 

Staining characteristics

   

Staining intensity

Moderate Weak Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Vesicles Cytoplasm, Mitochondria Cytoskeleton (Microtubules) 

Staining characteristics

   

Staining intensity

Weak Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Vesicles Mitochondria Cytoskeleton (Microtubules) 

Staining characteristics

   

Staining intensity

Weak Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & vesicles.
 

Immunofluorescent staining of human cell line A-431 shows positivity in mitochondria.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoskeleton (microtubules).
 

Summary

Staining of the nucleus and vesicles in all three cell lines.   Staining of mitochondria in all three cell lines with additional staining of cytoplasm in U-2 OS.   Staining of cytoskeleton (microtubules) in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Microtubules
Mitochondria
Nucleus but not nucleoli
Endosome