Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Golgi apparatus 

Staining summary

Mainly localized to the cytoplasm. In addition localized to the Golgi apparatus.

Reliability (APE)

Low

Antibodies in assay

HPA000980, CAB025797
 

Antibody HPA000980

 

Antibody CAB025797

 

         Cell line A-431

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Location(s)

Cytoplasm, Golgi apparatus  

Staining characteristics

Granular  

Staining intensity

Weak Negative 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm  

Staining characteristics

Granular  

Staining intensity

Weak Negative 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm  

Staining characteristics

Granular  

Staining intensity

Weak Negative 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & golgi apparatus.
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Summary

Staining of cytoplasm in all three cell lines. Additional staining of golgi in A-431.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Golgi apparatus