The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Cytoplasmic and nuclear expression in a subset of tissues. High expression in islets of Langerhans, prostate, Leydig cells and subsets of cells in seminiferus ducts.
Immunohistochemical staining of human kidney shows distinct nuclear and cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic and nuclear positivity in islet cells.
Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.
Summary
Strong cytoplasmic and nuclear staining was observed in the majority of normal cells. Chief cells in stomach were strongly positive while parietal cells were negative. Hepatocytes and smooth muscle cells were negative.
Most normal tissues displayed moderate cytoplasmic positivity, often combined with nuclear staining. Strong staining was observed in prostate, testis and pancreatic islet cells. Smooth muscle cells were negative.
Most normal tissues displayed moderate cytoplasmic positivity. Strong staining was observed in islet cells and leydig cells. Glial cells, smooth muscle, most squamous epithelia, ovarian and endometrial stromal cells were negative.
Most normal tissues showed weak cytoplasmic and/or nuclear positivity or were negative. Strong staining was observed in islets of Langerhans, the prostate, Leydig cells and subsets of cells in seminiferus ducts.
Weak to moderate cytoplasmic staining often combined with nuclear positivity was observed in most normal tissues. Leydig cells, purkinje cells and squamous epithelia except skin were strongly stained. Liver and glial cells were negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
