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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

Nucleus but not nucleoli 

Staining summary

Mainly localized to the cytoplasm and plasma membrane. In addition localized to the nucleus.

Reliability (APE)

High

Antibodies in assay

CAB007783, HPA022434, HPA022953, HPA022959, HPA028758
 

Antibody CAB007783

 

Antibody HPA022434

 

Antibody HPA022953

 

Antibody HPA022959

 

Antibody HPA028758

 

         Cell line A-431

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Location(s)

  Nucleus but not nucleoli, Plasma membrane, Cytoplasm Nucleus but not nucleoli, Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

      

Staining intensity

  Strong Strong Weak Weak 

Validation

  
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

      

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm Cytoplasm 

Staining characteristics

     

Staining intensity

Weak Weak Strong Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

     

         Cell line U-251 MG

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Location(s)

  Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm Nucleus but not nucleoli, Plasma membrane, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

      

Staining intensity

  Weak Weak Moderate Weak 

Validation

  
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

      

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of nucleus and cytoplasm in U-2 OS.   Staining of the cytoplasm and plasma membrane in all three cell lines. Additional staining of the nucleus in A-431.   Staining of the cytoplasm and plasma membrane in all three cell lines with additional staining of nucleus in A-431.   Staining of the cytoplasm and plasma membrane in all three cell lines. Additional staining of the nucleus in U-251 MG.   Staining of the cytoplasm in all three cell lines. Additional staining of the nucleus in U-251 MG and A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Plasma membrane