Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus, Plasma membrane 

Staining summary

Localized to nucleus, cytoplasm and plasma membrane.

Reliability (APE)

Medium

Antibodies in assay

HPA003162, CAB005191
 

Antibody HPA003162

 

Antibody CAB005191

 

         Cell line A-431

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Location(s)

 Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Nucleus, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Weak 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

   

Staining characteristics

   

Staining intensity

Negative   

Validation

No staining.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of plasma membrane and cytoplasm in A-431 and nucleus and cytoplasm in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus
Plasma membrane