The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Cells in red pulp of spleen, rectum and non-germinal cells in tonsil and lymph node displayed strong cytoplasmic and nuclear staining. Purkinje cells, heart muscle cells, oral mucosa, exocrine pancreas and stomach were moderately stained with additional nuclear or membranous staining in seminal vesicle and epididymis. Remaining normal tissues were weakly stained or negative.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Summary
Cells in red pulp of spleen, rectum and non-germinal cells in tonsil and lymph node displayed strong cytoplasmic and nuclear staining. Purkinje cells, heart muscle cells, oral mucosa, exocrine pancreas and stomach were moderately stained with additional nuclear or membranous staining in seminal vesicle and epididymis. Remaining normal tissues were weakly stained or negative.
Validation
One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data
