Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleoli, Golgi apparatus

Additional location(s)

Nucleus 

Staining summary

Mainly localized to nucleoli and the Golgi apparatus but also to nucleus.

Reliability (APE)

Low

Antibodies in assay

HPA013659, HPA015639
 

Antibody HPA013659

 

Antibody HPA015639

 

         Cell line A-431

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Location(s)

Nucleoli, Golgi apparatus  

Staining characteristics

  

Staining intensity

Weak Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleoli  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus, Nucleoli, Golgi apparatus  

Staining characteristics

Granular  

Staining intensity

Weak Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli.
 

Immunofluorescent staining of human cell line U-251MG shows no positivity.
 

Summary

Staining of Golgi apparatus and nucleoli in all three cell lines with additional staining of nucleus in U-251 MG.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Golgi apparatus
Nucleoli
Nucleus