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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Mitochondria

Additional location(s)

Nucleoli 

Staining summary

Staining of nucleus and mitochondria in all three cell lines with additional staining of nucleoli in U-251 MG and U-2 OS.

Reliability (Single)

Antibodies in assay

HPA023687
 

Antibody HPA023687

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Mitochondria 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleoli, Nucleus but not nucleoli, Mitochondria 

Staining characteristics

Spotty 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleoli, Nucleus but not nucleoli, Mitochondria 

Staining characteristics

Spotty 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & mitochondria.
 

Summary

Staining of nucleus and mitochondria in all three cell lines with additional staining of nucleoli in U-251 MG and U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Mitochondria
Nucleoli
Nucleus but not nucleoli