The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.
Immunohistochemical staining of human kidney shows distinct positivity in cells in glomeruli.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in glomeruli and endothlial cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.
Summary
Most of the normal tissues showed weak to moderate cytoplasmic and nuclear staining. Strong positivity was observed in bronchus, thyroid gland and renal glomeruli. Glial cells, lymphoid cells and squamous epithelia were mainly negative.
Distinct positivity was observed in heart muscle and endothelial cells. Renal glomeruli were strongly stained. Remaining normal tissues were in general negative.
Most of the normal tissues were negative. Endothelial cells, myoepithelium and renal glomeruli showed strong cytoplasmic positivity.
Glomeruli of kidney, myoepithelial cells of breast and endothelial cells exhibited strong immunoreactivity. Muscle tissues and peripheral nerve displayed moderate positivity. Remaining normal tissues were negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
