Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoskeleton (Intermediate filaments)

Additional location(s)

 

Staining summary

Localized to intermediate filaments.

Reliability (APE)

High

Antibodies in assay

CAB005889, HPA007007, HPA026111
 

Antibody CAB005889

 

Antibody HPA007007

 

Antibody HPA026111

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli     

Staining characteristics

     

Staining intensity

Weak     

Validation

Location not consistent with experimental gene/protein characterization data.
     

Comment

     

         Cell line U-2 OS

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Location(s)

Cytoskeleton (Intermediate filaments) Cytoskeleton (Intermediate filaments) Cytoskeleton (Intermediate filaments) 

Staining characteristics

   

Staining intensity

Strong Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Cytoskeleton (Intermediate filaments) Cytoskeleton (Intermediate filaments) 

Staining characteristics

   

Staining intensity

Weak Moderate Strong 

Validation

Location not consistent with experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments).
 

Immunofluorescent staining of human cell line U-251MG shows positivity in cytoskeleton (intermediate filaments).
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoskeleton (intermediate filaments).
 

Summary

Staining of nucleus in A-431 and U-251 MG and staining of cytoskeleton (intermediate filaments) in U-2 OS.   Staining of cytoskeleton (intermediate filaments) in U-251 MG and U-2 OS.   Staining of cytoskeleton (intermediate filaments) in U-251 MG and U- OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Intermediate filaments