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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoplasm

Additional location(s)

Nucleoli, Mitochondria 

Staining summary

Mainly localized to the nucleus and cytoplasm. Additionally localized to nucleoli and mitochondria.

Reliability (APE)

Very low

Antibodies in assay

HPA027218, HPA027381, HPA044837
 

Antibody HPA027218

 

Antibody HPA027381

 

Antibody HPA044837

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm   

Staining characteristics

    

Staining intensity

Weak Moderate   

Validation

The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
   

Comment

    

         Cell line U-2 OS

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Location(s)

Cytoplasm, Mitochondria Nucleus, Nucleoli, Cytoplasm   

Staining characteristics

    

Staining intensity

Weak Moderate   

Validation

The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
   

Comment

    

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus, Nucleoli, Cytoplasm  

Staining characteristics

   

Staining intensity

Moderate Moderate Negative 

Validation

The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
The multi-targeting antibody not yielding a staining pattern.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & mitochondria.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251 MG shows no positivity.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of nucleus in U-251 MG and A-431 as well as mitochondria in U-251 MG.   Staining of cytoplasm and nuclei in all three cell lines with additional staining of nucleoli in U-251 MG and U-2 OS.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Mitochondria
Nucleoli
Nucleus