The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human rectum shows distinct positivity in smooth muscle of vessels.
Immunohistochemical staining of human breast shows moderate positivity in myoepithelial cells.
Immunohistochemical staining of human breast shows strong positivity in myoepithelial cells.
Summary
Weak to moderate cytoplasmic positivity was observed in smooth muscle, myoepithelia, cells in stroma and vessels. Positivity was also observed in reaction center of tonsil. Other normal tissues were negative.
Urothelia and gall bladder showed strong granular positivity. Moderate cytoplasmic staining was observed in smooth muscle, myoepithelia, seminal vesicle and a subset of lymphoid cells. Other normal tissues were in general weakly stained or negative.
Myoepithelial cells in breast and salivary gland along with smooth muscle were strongly stained. moderate cytoplasmic positivity was observed in small intestine, ovary and leydig cells. Most other normal tissues were negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
