GGT2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA045635

 

Antibody HPA047534

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA045635 HPA047534 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

29 28 

Antigen sequence

LLPNVTTVERNIDQAVTAALETRHHHTQI
 
AVVQAIVRTAGGWAAASDSRKGGEPAGY
 

Matching transcripts

GGT2-001 - ENSP00000385601 [100%]
GGT2-002 - ENSP00000385721 [100%]
GGT2-201 - ENSP00000402035 [100%]
 GGT2-001 - ENSP00000385601 [100%]
GGT2-002 - ENSP00000385721 [100%]
GGT2-201 - ENSP00000402035 [100%]
 

Other gene match

GGT1 - ENSG00000100031 [100%]
GGTLC2 - ENSG00000100121 [100%]
GGTLC1 - ENSG00000149435 [97%]
GGTLC3 - ENSG00000183038 [100%]
 GGT1 - ENSG00000100031 [100%]
GGTLC2 - ENSG00000100121 [100%]
GGTLC1 - ENSG00000149435 [96%]
GGTLC3 - ENSG00000183038 [100%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human kidney shows strong luminal membranous positivity in cells in tubules.
More information
 Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in tubular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:150 1:400 

Literature conformity

Partly consistent with gene/protein characterization data No avaliable characterization data 

RNA consistency

Not done Not done 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

61.8, 61.4, 60.9, 27.0, 25.3, 24.3, 24.2, 24.1, 23.7, 23.6 61.8, 61.4, 60.9, 27.0, 25.3, 24.3, 24.2, 24.1, 23.7, 23.6 

Antibody dilution

1:250 1:370 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:2950 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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GGT2-001
 
GGT2-002
 
GGT2-201