CAMK1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA051409

 

Antibody CAB031904

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 

Product name

HPA051409 sc-1543 

Host species

Rabbit Goat 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Not known 

Released in version

12 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Synthetic peptide 

Length (aa)

21  

Antigen sequence

PRWKQAEDIRDIYDFRDVLGT
 
 

Matching transcripts

CAMK1-001 - ENSP00000256460 [100%]
CAMK1-003 - ENSP00000380447 [100%]
CAMK1-006 - ENSP00000404587 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

 Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in basal cells.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:65 

Validation IH

 Supportive: One antibody yielding a staining pattern which is consistent with gene/protein characterization data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

41.3, 14.0, 4.4 41.3, 28.5, 14.0, 4.4 

Antibody dilution

1:190 1:500 

Validation WB

Uncertain: Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line HEK 293 shows positivity in cytoplasm.
More information
  

Antibody dilution

1:38  

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:1550  

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).  
 

ANTIGEN VIEW

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CAMK1-001
 
CAMK1-003
 
CAMK1-006