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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Golgi apparatus

Additional location(s)

Nucleoli 

Staining summary

Staining of nucleus and Golgi apparatus in all three cell lines.

Reliability (Single)

Antibodies in assay

HPA028921
 

Antibody HPA028921

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Golgi apparatus 

Staining characteristics

 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus, Nucleoli, Golgi apparatus 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Golgi apparatus 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & golgi apparatus.
 

Summary

Staining of nucleus and Golgi apparatus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Golgi apparatus
Nucleoli
Nucleus