The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
A cytoplasmic expression in most tissues, most distinct in gastric glands, trophoblastic cells and a subset of glial cells.
Caution: Based on antibodies targeting proteins from multiple genes.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
Immunohistochemical staining of human cerebral cortex shows moderate positivity in a subset of glial cells
Immunohistochemical staining of human cerebral cortex shows moderate positivity in a subset of glial cells.
Summary
Fraction of glial cells and glandular cells in stomach displayed strong cytoplasmic positivity. Respiratory epithelia, epididymis, gall bladder, thyroid gland, urothelial cells, adrenal gland, placental trophoblasts, Leydig cells and endocervical glands were moderately stained. Remaining normal tissues were weakly stained or negative.
Normal tissues showed weak to moderate cytoplasmic staining. Salivary gland and Leydig cells exhibited strong positivity. Bile duct cells, thyroid, cells in glomeruli, skin, lymphoid and soft tissues were negative.
Most normal tissues displayed weak to moderate cytoplasmic staining. Adrenal cortical cells, trophoblastic cells and glandular cells of stomach exhibited strong cytoplasmic staining. Pneumocytes, bile duct cells, glomeruli, skeletal muscle and most lymphoid cells were negative.
Validation
The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein
The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein
The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein
