The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Cytoplasmic expression in many cell types, high expression levels in neuronal cells in hippocampus and cerebral cortex as well as glandular cells of stomach.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human hippocampus shows strong granular cytoplasmic positivity in neuronal cells.
Summary
Normal cells showed weak to moderate cytoplasmic positivity. Additional membranous staining was observed in a few glandular cells. Gastric chief cells, neuronal cells in hippocampus and cerebral cortex showed strong positivity. The liver, kidney and most squamous epithelia were mainly negative.
Most normal tissues showed weak to moderate cytoplasmic staining often with a granular pattern. Strong cytoplasmic positivity was seen in neuronal cells of the cerebral cortex and hippocampus and in glandular cells of gall bladder and stomach.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
