ACRV1

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA038718

 

Antibody HPA038719

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA038718 HPA038719 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

72 83 

Antigen sequence

TSSQPNELSGSIDHQTSVQQLPGEFFSLENPSDAEALYETSSGLNTLSEH
GSSEHGSSKHTVAEHTSGEHAE
 
SGEHASGEQASGAPISSTSTGTILNCYTCAYMNDQGKCLRGEGTCITQNS
QQCMLKKIFEGGKLQFMVQGCENMCPSMNLFSH
 

Matching transcripts

ACRV1-001 - ENSP00000432816 [100%]
ACRV1-002 - ENSP00000317684 [100%]
ACRV1-207 - ENSP00000407846 [100%]
 ACRV1-001 - ENSP00000432816 [100%]
ACRV1-003 - ENSP00000436819 [100%]
ACRV1-004 - ENSP00000433720 [100%]
ACRV1-201 - ENSP00000257382 [100%]
ACRV1-203 - ENSP00000257385 [100%]
ACRV1-206 - ENSP00000411583 [100%]
ACRV1-208 - ENSP00000412653 [100%]
ACRV1-002 - ENSP00000317684 [88%]
ACRV1-207 - ENSP00000407846 [88%]
ACRV1-209 - ENSP00000397448 [88%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferus ducts.
More information
 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferus ducts.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:300 1:35 

Validation IH

Supportive: Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data Supportive: Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412644)
More information
 

Target mass (kDa)

28.2, 26.4 28.2, 26.4, 22.4, 20.9, 19.1, 18.3, 17.8 

Antibody dilution

1:250 1:250 

Validation WB

Uncertain: No bands detected Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:500 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
ACRV1-001
 
ACRV1-002
 
ACRV1-003
 
ACRV1-004
 
ACRV1-201
 
ACRV1-203
 
ACRV1-206
 
ACRV1-207
 
ACRV1-208
 
ACRV1-209