The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human bronchus shows strong membranous and cytoplasmic positivity in respiratory epithelial cells.
Immunohistochemical staining of human bronchus shows strong membranous and cytoplasmic positivity in respiratory epithelial cells.
Immunohistochemical staining of human esophagus shows distinct nuclear and cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human nasopharynx shows strong cytoplasmic, nuclear and membranous positivity in respiratory epithelial cells.
Immunohistochemical staining of human bronchus shows strong positivity in respiratory epithelial cells.
Summary
Moderate to strong cytoplasmic positivity, in a few cases combined with membranous staining, was observed in salivary glands, lung, female genitalia, prostate, squamous and respiratory epithelia along with subsets of cells in lymphoid tissues. Cilia in respiratory epithelia and fallopian tube were distinctly stained. Remaining normal tissues were in general negative.
Strong cytoplasmic staining often combined with nuclear and membranous positivity was observed in bone marrow, bladder, placenta, ciliated cells, prostate, female genitalia and squamous epithelia. Remaining normal tissues were generally negative.
Normal tissues showed moderate to strong cytoplasmic and nuclear positivity. Cells of CNS were mostly negative.
The red pulp of spleen, placenta, poietic cells of bone marrow , squamous, urothelial, glandular cells in both genital and respiratory tract showed moderate to strong cytoplasmic and nuclear staining. Remaining normal cells were weakly stained or negative.
Most normal tissues exhibited moderate to strong cytoplasmic and nuclear positivity with additional membranous positivity in respiratory epithelia. Gastrointestinal tract, pancreas, liver, adrenal gland, testis, myocytes and cells in CNS were negative.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
