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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the plasma membrane and cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA011271, HPA011272, CAB013023, CAB035987
 

Antibody HPA011271

 

Antibody HPA011272

 

Antibody CAB013023

 

Antibody CAB035987

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm Cytoplasm Plasma membrane, Cytoplasm   

Staining characteristics

 Granular    

Staining intensity

Moderate Weak Strong   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
   

Comment

     

         Cell line U-2 OS

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Location(s)

   Plasma membrane, Cytoplasm Nuclear membrane, Plasma membrane, Cytoplasm 

Staining characteristics

     

Staining intensity

  Negative Strong Moderate 

Validation

  
No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

     

         Cell line U-251 MG

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Location(s)

Cytoplasm Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

Granular Granular   

Staining intensity

Weak Weak Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of cytoplasm and plasma membrane in A-431 and U-251 MG.   Staining of cytoplasm in U-251 MG and A-431 with additional staining of plasma membrane in U-251 MG.   Staining of plasma membrane and cytoplasm in all three cell lines.   Staining of plasma membrane and cytoplasm in U-251 MG. Staining of plasma membrane, cytoplasm and nuclear membrane in U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Plasma membrane