PWP1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA038708

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA038708 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

92 

Antigen sequence

MSLGKPAASLAVHTDKVQTLQFHPFEAQTLISGSYDKSVALYDCRSPDES
HRMWRFSGQIERVTWNHFSPCHFLASTDDGFVYNLDARSDKP
 

Matching transcripts

PWP1-001 - ENSP00000387365 [100%]
PWP1-005 - ENSP00000445249 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli.
More information
 

Antibody dilution

1:31 

Validation IF

Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

55.8, 49.0 

Antibody dilution

1:250 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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PWP1-001
 
PWP1-005