VILL

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA035675

 

Antibody HPA035676

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA035675 HPA035676 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

87 83 

Antigen sequence

IGWFFTWDPYKWTSHPSHKEVVDGSPAAASTISEITAEVNNLRLSRWPGN
GRAGAVALQALKGSQDSSENDLVRSPKSAGSRTSSSV
 
PKTSISEKARGLALTYSLRDRERGGGRAQIGVVDDEAKAPDLMQIMEAVL
GRRVGSLRAATPSKDINQLQKANVRLYHVYEKG
 

Matching transcripts

VILL-001 - ENSP00000283713 [100%]
VILL-002 - ENSP00000422096 [100%]
VILL-201 - ENSP00000373266 [100%]
 VILL-001 - ENSP00000283713 [100%]
VILL-201 - ENSP00000373266 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human colon shows strong luminal membrane positivity in glandular cells.
More information
 Immunohistochemical staining of human nasopharynx shows moderate membranous positivity in respiratory epithelial cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:150 1:5000 

Literature conformity

No avaliable characterization data No avaliable characterization data 

RNA consistency

Consistent with RNAseq data Mainly not consistent with RNAseq data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

95.9, 64.0 95.9 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in nucleus, nucleoli & cytoplasm.
More information
 

Antibody dilution

 1:182 

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available. 
 

ANTIGEN VIEW

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VILL-001
 
VILL-002
 
VILL-201