Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoskeleton (Intermediate filaments)

Additional location(s)

Nucleus, Plasma membrane, Centrosome 

Staining summary

Staining of cytoskeleton (intermediate filament) in U-2 OS. Staining of cytoskeleton (intermediate filament), nucleus and centrosome in U-251 MG. Staining of nucleus, plasma membrane and centrosome in A-431.

Reliability (Single)

Antibodies in assay

HPA037553
 

Antibody HPA037553

 

         Cell line A-431

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Location(s)

Nucleus, Plasma membrane, Centrosome 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Cytoskeleton (Intermediate filaments) 

Staining characteristics

 

Staining intensity

Strong 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Intermediate filaments), Centrosome 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments).
 

Summary

Staining of cytoskeleton (intermediate filament) in U-2 OS. Staining of cytoskeleton (intermediate filament), nucleus and centrosome in U-251 MG. Staining of nucleus, plasma membrane and centrosome in A-431.  

Validation

The subcellular location is not consistent with literature.
 

Dictionary

Centrosome
Intermediate filaments
Nucleus
Plasma membrane