The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
Summary
A majority of normal tissues displayed weak to moderate cytoplasmic positivity. Strong immunoreactivity was observed in colon, rectum, respiratory epithelial cells, adrenal gland and trophoblastic cells of placenta. Liver, prostate, pancreas, kidney and cells in CNS were negative.
A majority of normal tissues displayed weak to moderate cytoplasmic positivity. Strong immunoreactivity was observed in hepatocytes and pancreatic islet cells. Lymphoid tissues, urinary bladder, endometrium and fallopian tube were negative.
Normal tissues generally showed moderate cytoplasmic and nuclear positivity. The large intestine, placenta, subsets of lymphoid cells, thyroid and adrenal gland exhibited strong immunoreactivity. Glial cells of CNS were in general negative.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data
