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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules)

Additional location(s)

Vesicles 

Staining summary

Staining of nucleus and cytoskeleton (microtubules) in all three cell lines, with additional staining of vesicles in MCF-7.

Reliability (Single)

Antibodies in assay

HPA052408
 

Antibody HPA052408

 

         Cell line HeLa

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules) 

Staining characteristics

 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line MCF-7

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Location(s)

Nucleus but not nucleoli, Vesicles, Cytoskeleton (Microtubules) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules) 

Staining characteristics

 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoskeleton (microtubules).
 

Summary

Staining of nucleus and cytoskeleton (microtubules) in all three cell lines, with additional staining of vesicles in MCF-7.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Microtubules
Nucleus but not nucleoli
Endosome