Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleoli

Additional location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining summary

Staining of cytoplasm and nucleoli in all three cell lines with additional staining of plasma membrane in A-431 and nucleus in U-251 MG and U-2 OS.

Reliability (Single)

Antibodies in assay

CAB017112
 

Antibody CAB017112

 

         Cell line A-431

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Location(s)

Nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

Spotty 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleoli, Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Spotty 

Staining intensity

Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleoli, Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Spotty 

Staining intensity

Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleoli, nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm and nucleoli in all three cell lines with additional staining of plasma membrane in A-431 and nucleus in U-251 MG and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleoli
Nucleus but not nucleoli
Plasma membrane