Fields »

SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Focal Adhesions

Additional location(s)

Vesicles, Centrosome 

Staining summary

Staining of cytoplasm, plasma membrane and cell adhesions in A-431 and U-2 OS. Additional staining of vesicles and centrosome in U-251 MG.

Reliability (Single)

Antibodies in assay

HPA004748
 

Antibody HPA004748

 

         Cell line A-431

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Location(s)

Plasma membrane, Focal Adhesions 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Plasma membrane, Focal Adhesions 

Staining characteristics

Granular 

Staining intensity

Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Plasma membrane, Vesicles, Centrosome, Focal Adhesions 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & focal adhesions.
 

Summary

Staining of cytoplasm, plasma membrane and cell adhesions in A-431 and U-2 OS. Additional staining of vesicles and centrosome in U-251 MG.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Centrosome
Focal adhesions
Plasma membrane
Endosome