Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the nucleus (excluded from the nucleoli) but also localized to the cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA002038, HPA002698
 

Antibody HPA002038

 

Antibody HPA002698

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm   

Staining characteristics

Speckle   

Staining intensity

Moderate   

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
   

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm   

Staining characteristics

Granular   

Staining intensity

Moderate   

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
   

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Smooth  

Staining intensity

Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines.   Staining of cytoplasm and nucleus in U-251 MG.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli