The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human colon shows strong positivity in peripheral nerve/ganglion cells.
Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human bronchus shows strong membranous and cytoplasmic positivity in respiratory epithelial cells.
Summary
Most normal cells showed moderate or strong cytoplasmic positivity. Ovary, uterus, spleen, liver, endocrine glands and myocytes were weakly stained or negative.
Ciliated cells in respiratory epithelium and fallopian tubes showed moderate cytoplasmic positivity. Epididymis and parts of gastrointestinal tract, neuropil, neuronal cells in hippocampus and cells in seminiferus ducts exhibited weak to moderate staining. Other normal tissues were mainly negative.
Moderate cytoplasmic positivity was observed in most normal tissues. Respiratory epithelia and fallopian tube showed strong cytoplasmic and membranous staining. Strong cytoplasmic positivity was exhibited in germinal center cells, cells in seminiferus ducts of testis, epididymis and peripheral nerve. Hepatocytes, glial cells, placenta and muscle tissues were negative.
Validation
One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
The multi-targeting antibody yielding a staining pattern partly consistent with characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein chara
The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for at least one of the genes whereas any of the other genes has no available gene/protein characterization data or is contradicted by gene/protein
