Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm, Cytoskeleton (Intermediate filaments) 

Staining summary

Staining of nucleus in all three cell lines with additional staining of cytoplasm and cytoskeleton (intermediate filaments) in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA020053
 

Antibody HPA020053

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Cytoskeleton (Intermediate filaments) 

Staining characteristics

 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli 

Staining characteristics

 

Staining intensity

Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm & cytoskeleton (intermediate filaments).
 

Summary

Staining of nucleus in all three cell lines with additional staining of cytoplasm and cytoskeleton (intermediate filaments) in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Intermediate filaments
Nucleus but not nucleoli